Characterization of a novel endo-β-1,4-glucanase from Armillaria gemina and its application in biomass hydrolysis.
Identifieur interne : 002337 ( Main/Exploration ); précédent : 002336; suivant : 002338Characterization of a novel endo-β-1,4-glucanase from Armillaria gemina and its application in biomass hydrolysis.
Auteurs : Sujit Sadashiv Jagtap [Corée du Sud] ; Saurabh Sudha Dhiman ; Tae-Su Kim ; In-Won Kim ; Jung-Kul LeeSource :
- Applied microbiology and biotechnology [ 1432-0614 ] ; 2014.
Descripteurs français
- KwdFr :
- ADN fongique (composition chimique), ADN fongique (génétique), Analyse de séquence d'ADN (MeSH), Armillaria (classification), Armillaria (enzymologie), Armillaria (génétique), Armillaria (isolement et purification), Biomasse (MeSH), Cellulase (composition chimique), Cellulase (isolement et purification), Cellulase (métabolisme), Chromatographie sur gel (MeSH), Concentration en ions d'hydrogène (MeSH), Données de séquences moléculaires (MeSH), Espaceur de l'ADN ribosomique (composition chimique), Espaceur de l'ADN ribosomique (génétique), Hydrolyse (MeSH), Masse moléculaire (MeSH), Métabolisme glucidique (MeSH), Populus (métabolisme), Similitude de séquences d'acides aminés (MeSH), Stabilité enzymatique (MeSH), Température (MeSH).
- MESH :
- composition chimique : ADN fongique, Armillaria, Cellulase, Espaceur de l'ADN ribosomique.
- enzymologie : Armillaria.
- génétique : ADN fongique, Armillaria, Espaceur de l'ADN ribosomique.
- isolement et purification : Armillaria, Cellulase.
- métabolisme : Cellulase, Populus.
- Analyse de séquence d'ADN, Biomasse, Chromatographie sur gel, Concentration en ions d'hydrogène, Données de séquences moléculaires, Hydrolyse, Masse moléculaire, Métabolisme glucidique, Similitude de séquences d'acides aminés, Stabilité enzymatique, Température.
English descriptors
- KwdEn :
- Armillaria (classification), Armillaria (enzymology), Armillaria (genetics), Armillaria (isolation & purification), Biomass (MeSH), Carbohydrate Metabolism (MeSH), Cellulase (chemistry), Cellulase (isolation & purification), Cellulase (metabolism), Chromatography, Gel (MeSH), DNA, Fungal (chemistry), DNA, Fungal (genetics), DNA, Ribosomal Spacer (chemistry), DNA, Ribosomal Spacer (genetics), Enzyme Stability (MeSH), Hydrogen-Ion Concentration (MeSH), Hydrolysis (MeSH), Molecular Sequence Data (MeSH), Molecular Weight (MeSH), Populus (metabolism), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH), Temperature (MeSH).
- MESH :
- chemical , chemistry : Cellulase, DNA, Fungal, DNA, Ribosomal Spacer.
- classification : Armillaria.
- enzymology : Armillaria.
- genetics : Armillaria, DNA, Fungal, DNA, Ribosomal Spacer.
- isolation & purification : Armillaria, Cellulase.
- chemical , metabolism : Cellulase, Populus.
- Biomass, Carbohydrate Metabolism, Chromatography, Gel, Enzyme Stability, Hydrogen-Ion Concentration, Hydrolysis, Molecular Sequence Data, Molecular Weight, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Temperature.
Abstract
A novel endo-β-1,4-glucanase (EG)-producing strain was isolated and identified as Armillaria gemina KJS114 based on its morphology and internal transcribed spacer rDNA gene sequence. A. gemina EG (AgEG) was purified using a single-step purification by gel filtration. The relative molecular mass of AgEG by sodium dodecyl sulfate polyacrylamide gel electrophoresis was 65 kDa and by size exclusion chromatography was 66 kDa, indicating that the enzyme is a monomer in solution. The pH and temperature optima for hydrolysis were 5.0 and 60 °C, respectively. Purified AgEG had the highest catalytic efficiency with carboxymethylcellulose (k(cat)/K(m) = 3,590 mg mL⁻¹ s⁻¹) unlike that reported for any fungal EG, highlighting the significance of the current study. The amino acid sequence of AgEG showed homology with hydrolases from the glycoside hydrolase family 61. The addition of AgEG to a Populus nigra hydrolysate reaction containing a commercial cellulase mixture (Celluclast 1.5L and Novozyme 188) showed a stimulatory effect on reducing sugar production. AgEG is a good candidate for applications that convert lignocellulosic biomass to biofuels and chemicals.
DOI: 10.1007/s00253-013-4894-x
PubMed: 23604561
Affiliations:
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(chemistry)</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Enzyme Stability (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Molecular Weight (MeSH)</term><term>Populus (metabolism)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (composition chimique)</term><term>ADN fongique (génétique)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Armillaria (classification)</term><term>Armillaria (enzymologie)</term><term>Armillaria (génétique)</term><term>Armillaria (isolement et purification)</term><term>Biomasse (MeSH)</term><term>Cellulase (composition chimique)</term><term>Cellulase (isolement et purification)</term><term>Cellulase (métabolisme)</term><term>Chromatographie sur gel (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Espaceur de l'ADN ribosomique (composition chimique)</term><term>Espaceur de l'ADN ribosomique (génétique)</term><term>Hydrolyse (MeSH)</term><term>Masse moléculaire (MeSH)</term><term>Métabolisme glucidique (MeSH)</term><term>Populus (métabolisme)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Stabilité enzymatique (MeSH)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulase</term><term>DNA, Fungal</term><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Armillaria</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN fongique</term><term>Armillaria</term><term>Cellulase</term><term>Espaceur de l'ADN ribosomique</term></keywords><keywords scheme="MESH" qualifier="enzymologie" 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xml:lang="en"><term>Biomass</term><term>Carbohydrate Metabolism</term><term>Chromatography, Gel</term><term>Enzyme Stability</term><term>Hydrogen-Ion Concentration</term><term>Hydrolysis</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Biomasse</term><term>Chromatographie sur gel</term><term>Concentration en ions d'hydrogène</term><term>Données de séquences moléculaires</term><term>Hydrolyse</term><term>Masse moléculaire</term><term>Métabolisme glucidique</term><term>Similitude de séquences d'acides aminés</term><term>Stabilité enzymatique</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel endo-β-1,4-glucanase (EG)-producing strain was isolated and identified as Armillaria gemina KJS114 based on its morphology and internal transcribed spacer rDNA gene sequence. A. gemina EG (AgEG) was purified using a single-step purification by gel filtration. The relative molecular mass of AgEG by sodium dodecyl sulfate polyacrylamide gel electrophoresis was 65 kDa and by size exclusion chromatography was 66 kDa, indicating that the enzyme is a monomer in solution. The pH and temperature optima for hydrolysis were 5.0 and 60 °C, respectively. Purified AgEG had the highest catalytic efficiency with carboxymethylcellulose (k(cat)/K(m) = 3,590 mg mL⁻¹ s⁻¹) unlike that reported for any fungal EG, highlighting the significance of the current study. The amino acid sequence of AgEG showed homology with hydrolases from the glycoside hydrolase family 61. The addition of AgEG to a Populus nigra hydrolysate reaction containing a commercial cellulase mixture (Celluclast 1.5L and Novozyme 188) showed a stimulatory effect on reducing sugar production. AgEG is a good candidate for applications that convert lignocellulosic biomass to biofuels and chemicals.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23604561</PMID><DateCompleted><Year>2014</Year><Month>08</Month><Day>29</Day></DateCompleted><DateRevised><Year>2014</Year><Month>01</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0614</ISSN><JournalIssue CitedMedium="Internet"><Volume>98</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Characterization of a novel endo-β-1,4-glucanase from Armillaria gemina and its application in biomass hydrolysis.</ArticleTitle><Pagination><MedlinePgn>661-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00253-013-4894-x</ELocationID><Abstract><AbstractText>A novel endo-β-1,4-glucanase (EG)-producing strain was isolated and identified as Armillaria gemina KJS114 based on its morphology and internal transcribed spacer rDNA gene sequence. A. gemina EG (AgEG) was purified using a single-step purification by gel filtration. The relative molecular mass of AgEG by sodium dodecyl sulfate polyacrylamide gel electrophoresis was 65 kDa and by size exclusion chromatography was 66 kDa, indicating that the enzyme is a monomer in solution. The pH and temperature optima for hydrolysis were 5.0 and 60 °C, respectively. Purified AgEG had the highest catalytic efficiency with carboxymethylcellulose (k(cat)/K(m) = 3,590 mg mL⁻¹ s⁻¹) unlike that reported for any fungal EG, highlighting the significance of the current study. The amino acid sequence of AgEG showed homology with hydrolases from the glycoside hydrolase family 61. The addition of AgEG to a Populus nigra hydrolysate reaction containing a commercial cellulase mixture (Celluclast 1.5L and Novozyme 188) showed a stimulatory effect on reducing sugar production. AgEG is a good candidate for applications that convert lignocellulosic biomass to biofuels and chemicals.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jagtap</LastName><ForeName>Sujit Sadashiv</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dhiman</LastName><ForeName>Saurabh Sudha</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Tae-Su</ForeName><Initials>TS</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>In-Won</ForeName><Initials>IW</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jung-Kul</ForeName><Initials>JK</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>JF825075</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>04</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D055392" MajorTopicYN="N">Armillaria</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002850" MajorTopicYN="N">Chromatography, Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004795" MajorTopicYN="N">Enzyme Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>01</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>4</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>4</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>8</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23604561</ArticleId><ArticleId IdType="doi">10.1007/s00253-013-4894-x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Dhiman, Saurabh Sudha" sort="Dhiman, Saurabh Sudha" uniqKey="Dhiman S" first="Saurabh Sudha" last="Dhiman">Saurabh Sudha Dhiman</name><name sortKey="Kim, In Won" sort="Kim, In Won" uniqKey="Kim I" first="In-Won" last="Kim">In-Won Kim</name><name sortKey="Kim, Tae Su" sort="Kim, Tae Su" uniqKey="Kim T" first="Tae-Su" last="Kim">Tae-Su Kim</name><name sortKey="Lee, Jung Kul" sort="Lee, Jung Kul" uniqKey="Lee J" first="Jung-Kul" last="Lee">Jung-Kul Lee</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Jagtap, Sujit Sadashiv" sort="Jagtap, Sujit Sadashiv" uniqKey="Jagtap S" first="Sujit Sadashiv" last="Jagtap">Sujit Sadashiv Jagtap</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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